Plant Tissue Culture

enjoy in this websitePlant Tissue Culture
Presenter: Lydiane (Ann) Kyte
Host: Kathy Liu
Discussion
Did you ever have a plant that was so unique or so beautiful that you wished you had hundreds or thousands of them to enjoy or to sell? Plant tissue culture (micropropagation) is a technique which will do just that for us. We are going to discuss this tool which is used so extensively in the nursery business and in plant biotechnology. It is a fascinating and useful tool which allows the rapid production of many genetically identical plants using relatively small amounts of space, supplies and time.

Basically the technique consists of taking a piece of a plant (such as a stem tip, node, meristem, embryo, or even a seed) and placing it in a sterile, (usually gel-based) nutrient medium where it multiplies. The formulation of the growth medium is changed depending upon whether you are trying to get the plant to produce undifferentiated callus tissue, multiply the number of plantlets, grow roots, or multiply embryos for “artificial seed”.

For many who become superficially aware of the technique it seems shrouded in mystery and is shrugged off as too technical to be of concern. Actually, it is no more of a mystery than taking a cutting of your favorite house plant and growing it to share with a friend. As for being technical, you can begin plant tissue culture with as little as a cookbook approach and a feeling for sterile technique.

Some people have visions of scientists doing plant tissue cultures in white gowns and masks in hospital-clean environments. Such conditions are excessive. While it is true that mold spores, bacteria, and other contaminants will grow and overrun a culture, air that is not moving has a minimum of contaminants. In addition, disinfection of implements, work surface and nearby areas helps eliminate contaminants.

The guidelines for preparation and the laboratory protocol provided here are given as a place to begin. Included with is a limited discussion of some of the many options you have as you explore micropropagation. We can discuss these in more depth if you have questions, concerns or related experiences to share. I would be particularly interested in success and challenges you may have had or are currently having in your classroom.

Some suggestions are given for the following
(a) Selecting plant sources. Some species, or even clones are easier to grow in culture than others. Some respond reluctantly to culture, some do not respond at all, and many plants have never been tried.

(b) Choosing a growth medium (price, convenience, type of plant and purpose of the micropropagation all enter into this decision.) How important are the kinds of hormones used? On limited scale, media ingredients are available at the grocery and health food stores.

(c) Suggestions for media preparation and sterilization. There are alternatives to sterilization in a pressure cooker or an autoclave.

(d) Methods for cleaning, storing and manipulating explants (plant pieces to be cultured).

Given certain basics there are many options for procedure, equipment and supplies for plant tissue culture. Some of your decisions will be based upon the amount of time, money and space you have. Other decisions will be based upon why you are doing plant tissue culture and what you expect as a result (more plants?) . Catalogs, such as Sigma, Carolina Biological, or Edmund Scientific are good reference and they are for purchasing needed materials..

I look forward to sharing tissue culture experiences with you.

References:
Debergh, P.C. and R.H. Zimmerman, eds. 1991. Micropropagation, Technology and Application. Kluwer Academic Publishers. $61.50. Lab design, info on labs worldwide, in depth discussions of problems. Not for the beginner.

Donnelly, D.J., and W.E.Vidaver, 1988. Glossary of Plant Tissue Culture, Portland, OR. Timber Press, $22.95. Good definitions of tissue culture terms.

Kyte, Lydiane and J. Kleyn, 1996. Plants from Test Tubes: An Introduction to Micropropagation, 3rd ed., Timber Press, 1996 $29.95. Good basics for the beginning amateur or grower.

Smith, Roberta H., 1992. Plant Tissue Culture-Techniques and Experiments. Academic Press. $35.00. Good introduction and broad base for college course.

Trigiano, Robert N, and Dennis J. Gray, eds.1996,Plant Tissue Culture Concepts and Laboratory Exercises. CRC Press. $65.00. For the advanced student.

Sources of supplies:

Carolina Biological

Edmond Scientific

PhytoTechnology Laboratories
This company specializes in plant tissue culture supplies. Downloadable documents (choose MS Word or PDF format) on Media Preparation, Setting Up a Tissue Culture Lab, Basic Laboratory Procedures, and more are available in the “Technical” section of the web site.

Sigma, 1996. “Plant Tissue Culture Catalog”.
In addition to media ingredients, premixes, equipment and supplies, this catalog contains a media comparison chart, procedures for media preparations.references and other valuable data.

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Plant Tissue Culture Media
Plant Tissue Culture Media

Plant Tissue Culture refers to the technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium. Culture media used for in vitro cultivation of plant cells are composed of following basic components:

Complex Mixture of Salts: Essential elements, or mineral ions

Organic Supplement: vitamins and/or amino acids

Carbon Source: usually sugar sucrose

Gelling Agents

Plant Growth Regulators

Antibiotics
Complex Mixture of Salts
These include essential elements or mineral ions important for plant nutrition and their physiological function. The essential elements can further be divided into the following categories:

Macroelements (or macronutrients)
Microelements (or micronutrients)
Iron source

Macroelements :- These elements are required in large amounts for plant growth and development. Nitrogen, phosphorus, potassium, magnesium, calcium and sulphur (and carbon, which is added separately) are regarded as macroelements. These elements comprise at least 0.1% of the dry weight of plants.

Microelements :- These elements are required in trace amounts for plant growth and development. Manganese, iodine, copper, cobalt, boron, molybdenum, iron and zinc are regarded as microelements, although other elements like aluminium and nickel are frequently found in some formulations.

Plant Tissue Culture Media

Iron Source :- Iron is usually added in the medium as iron sulphate, although iron citrate can also be used. Ethylenediaminetetraacetic acid (EDTA) is usually used in conjunction with the iron sulphate. The EDTA complexes with the iron so as to allow the slow and continuous release of iron into the medium. Uncomplexed iron can precipitate out of the medium as ferric oxide.

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Organic Supplements
These include vitamins and amino acids. Two vitamins, i.e., thiamine (vitamin B1) and myoinositol (a vitamin B) are essential for the culture of plant cells in vitro. However, other vitamins are often added to for historical reasons. The most commonly used amino acid is glycine. However, arginine, asparagine, aspartic acid, alanine, glutamic acid, glutamine and proline are also used. Amino acids provide a source of reduced nitrogen and, like ammonium ions, uptake causes acidification of the medium. Casein hydrolysate can be used as a source of a mixture of amino acids.

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Carbon Source
The most commonly used carbon source is sucrose. It is readily assimilated and relatively stable. Other carbohydrates like glucose, maltose, galactose and sorbitol can also be used and may prove better than sucrose in specialized circumstances.
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Gelling Agents
Plant tissue culture media can be used in either liquid or ‘solid’ forms, depending on the type of culture being grown. Agar, produced from seaweed, is the most common type of gelling agent, and is ideal for routine applications. For more demanding applications, a range of purer gelling agents are available. Purified agar or agarose can be used, as can a variety of gellan gums.

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Plant Growth Regulators
Specific media manipulations can be used to direct the development of plant cells in culture due to plasticity and totipotency. Plant growth regulators are the critical media components in determining the developmental pathway of the plant cells. There are five main classes of plant growth regulator used in plant cell culture, namely:

Auxins

Cytokinins

Gibberellins

Abscisic Acid

Ethylene

Auxins

:-

Auxins promote both cell division and cell growth. IAA (indole-3-acetic acid) is the most important naturally occurring auxin but its use in plant tissue culture media is limited because it is unstable to both heat and light. 2,4-Dichlorophenoxyacetic acid (2,4-D) is the most commonly used auxin and is extremely effective in most circumstances.

Cytokinins

:-

Cytokinins promote cell division. Of the naturally occurring cytokinins, only zeatin and 2iP (2-isopentyl adenine have some use in plant tissue culture media. The synthetic analogues, kinetin and BAP (benzylaminopurine), are used more frequently. Non-purine-based chemicals, such as substituted phenylureas, are also used as cytokinins in plant tissue culture media.

Auxins

:-

Auxins promote both cell division and cell growth. IAA (indole-3-acetic acid) is the most important naturally occurring auxin but its use in plant tissue culture media is limited because it is unstable to both heat and light. 2,4-Dichlorophenoxyacetic acid (2,4-D) is the most commonly used auxin and is extremely effective in most circumstances.

Plant Tissue Culture Media

Cytokinins

:-

Cytokinins promote cell division. Of the naturally occurring cytokinins, only zeatin and 2iP (2-isopentyl adenine have some use in plant tissue culture media. The synthetic analogues, kinetin and BAP (benzylaminopurine), are used more frequently. Non-purine-based chemicals, such as substituted phenylureas, are also used as cytokinins in plant tissue culture media.

Gibberellins

:-

Gibberellins are involved in regulating cell elongation, in determining plant height and fruit-set. Only a few of the gibberellins like GA3 are used in plant tissue culture media.
Abscisic Acid

:-

It is used in plant tissue culture to promote distinct developmental pathways such as somatic embryogenesis. Abscisic acid (ABA) inhibits cell division.

Ethylene

:-

Ethylene is associated with controlling fruit ripening in climacteric fruits, and its use in plant tissue culture is not widespread. Some plant cell cultures produce ethylene, which, if it builds up sufficiently, can inhibit the growth and development of the culture.

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Antibiotics
Antibiotics are substances produced by certain microorganisms that suppress the growth of other microorganisms and eventually destroy them. Their applications include:
A.

Suppresses bacterial infections in plant cell and tissue culture.
B.

Suppresses mould and yeast infections in cell cultures.
C.

Eliminates Agrobacterium species after the transformation of plant tissue.
These antibiotics can be divided into different classes on the basis of chemical structure and their mechanism of action:

Inhibitors of Bacterial Cell Wall Synthesis

e.g. β-lactam antibiotics, Penicillins and Cephalosporins.

Antibiotics that affect Cell Membrane permeability.

• Antibacterial e.g. Colistin Sulphate, Polymixin B Sulphate, Gramicidin
• Antifungal e.g. Amphotericin B, Nystatin, Pimaricin

Bacteriostatic Inhibitors of Protein

Plant Tissue Culture Media

Antibiotics that affect the function of 30 S or 50 S ribosomal subunits to cause a reversible inhibition of protein synthesis. e.g. Chloramphenicol, Chlortetracycline HCl, Clindamycin HCl, Doxycycline HCl, Erythromycin, Lincomycin HCl, Oxytetracycline HCl, Spectinomycin sulphate, Tetracycline HCl, Tylosin tartrate, Lincomycin HCl

Bactericide Inhibitors of Protein Synthesis

Antibiotics that bind to the 30 S ribosomal subunit and alter protein synthesis which eventually leads to cell death. This group includes:

* Aminoglycosides: Apramycin, Butirosine, Gentamicin, Kanamycin, Neomycin, Streptomycin,
Tobramycin.
* Inhibitors of Nucleic Acid Metabolism: e.g. Rifampicin, Mitomycin C and Nalidixic acid
* Antimetabolites: Antibiotics, which block specific metabolic steps that are essential to microorganisms
e.g. Metronidazole, Miconazole, Nitrofurantoin, Trimethoprim and Sulphomethoxazole.
* Nucleic Acid Analogs, which inhibit enzymes essential for DNA synthesis. e.g. 5-Fluorouracil, Mercaptopurine.

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Preparation of Plant Tissue Culture Medium

Measure approximately 90% of the required volume of the deionized-distilled water in a flask/container of double the size of the required volume.

Add the dehydrated medium into the water and stir to dissolve the medium completely. Gentle heating of the solution may be required to bring powder into solution.

Add desired heat stable supplements to the medium solution.

Add additional deionized-distilled water to the medium solution to obtain the final required volume.

Set the desired pH with NaOH or HCl.

Dispense the medium into culture vessels.

Sterilize the medium by autoclaving at 15 psi (121οC) for appropriate time period. Higher temperature

may result in poor cell growth.

Add heat labile supplements after autoclaving.

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RUMUS – RUMUS 20 ASAM AMINO DASAR

RUMUS
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Bid. Peminatan: Kultur Jaringan Tanaman
C. RUMUS – RUMUS 20 ASAM AMINO DASAR
1. Glisina (Asam 2-aminoetanoat)
(C2H5NO2)
2. Alanina (Asam (S)-2-aminopropanoat)
(C3H7NO2)
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3. Valina (Asam S-2-amino-3-metil-butanoat)
(C5H11NO2)
4. Leusina (Asam S-2-amino-4-metil-pentanoat)
(C6H13NO2)
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5. Isoleusina (Asam 2S,3S-2-amino-3-metilpentanoat)
(C6H13NO2)
6. Serina (Asam S-2-amino-3-hidroksipropanoat)
(C3H7NO3)
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7. Treonina (Asam2S,3R-2-amino-3-hidroksibutanoat)
(C4H9NO3)
8. Asam aspartat (Asam 2S-2-aminobutandioat)
(C4H7NO4)
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9. Asam glutamat (Asam 2S-2-aminopentandioat)
(C5H9NO4)
10. Asparagina (Asam 2S-2-amino-3-karbamoil- propanoat)
(C4H8N2O3)
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11. Glutamina (Asam 2S-2-amino-4-karbamoil-butanoat)
(C5H10N2O3)
12. Arginina (AsamS-2-amino-5-(diamino metilidenamino) pentanoat)
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(C6H14N4O2)
13. Histidina (AsamS-2-amino-3-(3H-imidazol-4-il)propanoat)
(C6H9N3O2)
14. Lisina (Asam S-2,6-diaminoheksanoat)
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(C6H14N2O2)
15. Sisteina (Asam 2R-2-amino-3-sulfanil-propanoat)
(C3H7NO2S1)
16. Metionina (AsamS-2-amino-4-(metilsulfanil)-butanoat)
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(C5H11NO2S)
17. Prolina (AsamS-pirolidin-2-karboksilat)
(C5H9NO2)
18. Fenilalanina (Asam 2-amino-3-fenil-propanoat)
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(C9H11NO2)
19. Tirosina (Asam S-2-amino-3-(4-hidroksi- fenil)-propanoat)
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(C9H11NO3)
20. Triptofan (Asam S-2-amino-3-(1H-indol-3-il)-propanoat)
(C11H12N2O2)
Daftar Pustaka
http://www.biology.arizona.edu\biochemistry\biochemistry.html, 2003, The Biology Project-
Biochemistry. [Webpage (Downloads: Rabu,11 Maret.2009)]
Wapedia – Wiki. 2008. Asam amino. [Webpage (Downloads: Rabu,11 Maret.2009)]
Wikipedia .2009. Asam amino. [Webpage (Downloads: Rabu,11 Maret.2009)]

PROTEIN DALAM TUMBUHAN

asalamualaikum sobat semoga kita semua tetap dalam rahmat Allah amin.

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D. PROTEIN DALAM TUMBUHAN
1. Pada bagian mana protein pada tumbuhan ditemukan?
Informasi didalam gen ditentukan oleh rangkaian linear nukleotida pada DNA. Suatu gen
tunggal mungkin mempunyai panjang ratusan ribu nukleotida. DNA tidak membentuk
protein secara langsung, DNA memberikan perintah perangkat sintesis protein dalam bentuk
RNA. Proses pembentukan salinan RNA dari DNA adalah transkipsi. Setelah DNA
ditranskripsikan di dalam nukleus RNA bergerak ke sitoplasma. Proses penggunaan
informasi di RNA untuk menyintesis protein adalah translasi. Dan translasi berlangsung di
sitoplasma.
Bila ditanyakan dimana protein itu berada pada tanaman maka jawabannnya adalah
Protein pada bagian tubuh tanaman terdapat hampir dalam seluruh bagian tubuh tumbuhan.
Protein ditemukan pada daun muda dan pada bagian tubuh lainnya seperti polong, dan buah .
Protein adalah senyawa organik kompleks berbobot molekul tinggi yang merupakan polimer dari
monomer-monomer asam amino yang dihubungkan satu sama lain dengan ikatan peptida.
Tumbuhan menyerap unsur-unsur hara dalam tanah melalui akar dan disalurkan
keseluruh bagian tanaman sampai ke daun sehingga tumbuhan membentuk protein dan
melakukan perombakan (proses katabolisme). Nitrogen berperan dalam pembentukan sel ,
jaringan , dan organ tanaman. Ia berfungsi sebagai sebagai bahan sintetis klorofil , protein ,
dan asam amino. Karena itu kehadirannya dibutuhkan dalam jumlah besar , terutama saat
pertumbuhan vegetatif. Dalam unsur-unsur tersebut mengandung unsure Nitrogen yang
merupakan unsure pembentuk pada protein. Unsur Nitrogen yang terdapat pada protein
adalah 16% dari protein tersebut. Yang banyak tersimpan pada pucuk dan daun muda. Dan
masih banyak lagi unsur-unsur yang merupakan pembentuk dari protein yang tersedia pada
tumbuhan.
Beberapa penelitian menunjukkan keberadaan protein yang memiliki letak berbeda-beda
pada tumbuhan. Pada famili serealia seperti gandum, padi, polong – polongan dan jagung
protein berada pada bagian bijinya. Pada tanaman tembakau, protein banyak ditemukan
dibagian daunnya. Sedangkan pada kantong semar, protein banyak ditemukan pada bagian
antara batang dengan bunga, selain itu pada buah petai terdapat kandungan protein yang
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tinggi . Terbentuknya protein bermula dari proses anabolisme dan kemudian dirombak pada
tumbuhan tersebut melalui proses katabolisme.
Pada tumbuhan protein dapat dilihat dari kandungan Nitrogen pada tumbuhan.
Kandungan Nitrogen merupakan unsur yang dominan mempengaruhi pertumbuhan tanaman
tersebut. Sehingga tanaman sangat memerlukan Nitrogen untuk pembentukan protein pada
tanaman dan apabila kekurangan Nitrogen dapat diartikan sebagai kekurangan protein.
2. Sebutkan jenis proteinnya?
Protein yang terdapat pada makhluk hidup memiliki jenis yang berbeda dan memiliki
fungsi masing-masing. Protein yang dihasilkan oleh tumbuhan berbeda – beda dapat
dicontohkan pada beberapa tanaman sebagai berikut:
Protein prolamin banyak terdapat pada tanaman biji-bijian/sereal seperti beras polongpolongan
dan jagung, tidak memiliki lysine. Pada tanaman polong/kacang-kacangan yang
mayoritas mengandung protein yaitu protein globulin, kekurangan cysteine, dan
methionine. Protein ini memiliki asam amino yang esensial. Selain itu Menurut Davidson,
tanaman memiliki unsur kimia yang dapat melindungi mereka dari herbivora pemakan daun
seperti jenis serangga tertentu dan monyet.hal ini menunjukan bahwa tanaman memiliki
kandungan protein sebagai pelindung dirinya dari serangan organism pengganggu.
3. Bagaimana ciri-ciri tumbuhan yang kekurangan protein?
Kekurangan protein pada tanaman sama dengan kekurangan Nitrogen karena pada
tanaman terdapat 16% Nitrogen penyusun protein gejala kekurangannya yaitu:
a. Tanaman tumbuh kerdil,
b. Daun menguning karena kekurangan klorofil. Lebih lanjut mengering dan rontok.
c. Tulang-tulang di bawah permukaan daun muda tampak pucat.
d. Pertumbuhan tanaman lambat , kerdil dan lemah.
e. Produksi bunga dan biji rendah.
f. Jaringan tanaman mengering dan mati,
g. Tanaman akan mati atau kering apabila tidak diatasi

5 KUNCI PENGOKOH JIWA PENENANG BATHIN DALAM MENGARUNGI PERSOALAN HIDUP

5 KUNCI PENGOKOH JIWA PENENANG BATHIN
DALAM MENGARUNGI PERSOALAN HIDUP
Oleh KH. Abdullah Gymnastiar (AA Gym)

Salam Hikmah
Assalaamu’alaikum wa rahmatullahi wa barakaatuh
Salam sejahtera untuk kita semua

Sahabat Hikmah…
Hidup ini sebenarnya bukan kehidupan yang sebenarnya…
Hidup ini hanyalah ARENA UJIAN diri,
agar Allah memilih untuk KEHIDUPAN YANG SEBENARNYA,
siapa-siapa yang menjadi HAMBA ALLAH dan menjadi penghuni Surga serta bertemu dengan Tuhannya,
dan siapa-siapa yang menjadi HAMBA IBLIS dan menjadi penghuni Neraka serta bertemu dengan Tuhannya,
Sehingga dalam mengarungi ujian ini kita akan menghadapi berbagai persoalan hidup…
Tidak mungkin hidup di dunia tanpa persoalan…

” Allah Yang menjadikan mati dan hidup, supaya Dia MENGUJI kamu, siapa di antara kamu yang LEBIH BAIK perbuatannya. Dan Dia Maha Perkasa lagi Maha Pengampun.” (QS 67:2)

Agar jiwa kita tenang dan kokoh menghadapi persoalan hidup, berikut ini ada tips dari Aa Gym yang layak untuk diikuti:

1. AKU HARUS SIAP MENGHADAPI HIDUP INI, APAPUN YANG TERJADI

• Hidup di dunia ini hanya satu kali, aku tak boleh gagal dan sia-sia tanpa guna

• Tugasku adalah menyempurnakan niat dan ikhtiar, perkara apapun yang terjadi kuserahkan kepada Allah Yang Maha Tahu yang terbaik bagiku

• Aku harus selalu sadar sepenuhnya bahwa yang terbaik menurutku belum tentu yang terbaik menurut Allah SWT. Bahkan sangat mungkin aku terkecoh oleh keinginan dan harapanku sendiri

• Pengetahuan tentang diriku atau tentang apapun amat terbatas sedangkan pengetahuan Allah menyelimuti segalanya, Dia tahu awal, akhir dan segala-galanya

• Sekali lagi betapapun aku sangat menginginkan sesuatu, tetap hatiku harus kupersiapkan untuk menghadapi kenyataan yang tak sesuai dengan harapanku. Karena mungkin itulah yang terbaik bagiku

2. AKU HARUS RELA DENGAN KENYATAAN YANG TERJADI

• Bila sesuatu terjadi, yaa….. inilah kenyataan dan episode hidup yang harus kujalani

• Aku harus menikmatinya, dan aku tak boleh larut dalam kekecewaan berlama-lama, kecewa, dongkol, sakit hati tak akan merobah apapun selain menyengsarakan diriku sendiri, dongkol begini, tak dongkol juga tetap begini

• Hatiku harus realistis menerima kenyataan yang ada, namun tubuh serta pikiranku harus tetap bekerja keras mengatasi dan menyelesaikan masalah ini

• Bila nasi telah menjadi bubur, maka aku harus mencari ayam, cakweh, kacang polong, kecap, seledri, bawang goreng dan sambal agar bubur ayam spesial tetap dapat kunikmati

3. AKU TAK BOLEH MEMPERSULIT DIRI

• Aku harus yakin bahwa hidup ini bagai siang dan malam pasti silih berganti. Tak mungkin siang terus-menerus dan tak mungkin juga malam terus-menerus, pasti setiap kesenangan ada ujungnya begitupun masalah yang menimpaku pasti ada akhirnya, aku harus sangat sabar menghadapinya

• Akupun harus yakin bahwa setiap musibah terjadi dengan ijin Allah Yang Maha Adil, pasti sudah diukur dengan sangat cermat oleh-Nya tak mungkin melampaui batas kemampuanku, karena Dia tak pernah mendzolimi hamba-hamba-Nya

• Aku tak boleh mendzolimi diriku sendiri, dengan pikiran buruk yang mempersulit dan menyengsarakan diri, pikiranku harus tetap jernih, terkendali, tenang dan proporsional, aku tak boleh terjebak mendramatisir masalah

• Aku harus berani menghadapi persoalan demi persoalan, tak boleh lari dari kenyataan, karena lari sama sekali tak menyelesaikan bahkan sebaliknya hanya akan menambah masalah. Semua harus dengan tegar kuhadapi dengan baik, aku tak boleh menyerah, aku tak boleh kalah

• Mesti segala sesuatu akan ada akhirnya, begitupun persoalan yang kuhadapi seberat apapun seperti yang dijanjikan Allah ” Fainnama’al usri yusron innama’al ’usri yusron” dan sesungguhnya bersama kesulitan itu pasti ada kemudahan, bersama kesulitan itu pasti ada kemudahan. Janji yang tak pernah mungkin dipungkiri oleh Allah SWT

4. EVALUASI DIRI

• Segala yang terjadi mutlak adalah ijin Allah SWT, dan Allah tak mungkin berbuat sesuatu yang sia-sia

• Pasti ada hikmah dibalik setiap kejadian, sepahit apapun pasti ada kebaikan yang terkandung didalamnya, bila disikapi dengan sabar dan benar

• Harus kurenungkan mengapa Allah menakdirkan semua ini menimpaku, bisa jadi peringatan atas dosa-dosa kita, kelalaianku atau mungkin, saat kenaikan kedudukanku disisi Allah

• Mungkin aku harus berpikir keras untuk menemukan kesalahan yang kuperbaiki

• Setiap kejadian bagai cermin pribadiku, aku tak boleh gentar dengan kekurangan dan kesalahan yang telah terjadi, yang penting kini aku mengetahui diriku yang sebenarnya dan aku bertekad sekuat tenaga untuk memperbaikinya, Allah Maha Pengampun dan Maha Penerima Taubat

5. ALLAH LAH SATU-SATUNYA PENOLONGKU

• Aku harus yakin kalaupun bergabung seluruh manusia dan jin untuk menolongku tak mungkin terjadi apapun tanpa ijin-Nya

• Hatiku harus bulat total dan yakin seyakin-yakinnya, bahwa hanya Allahlah satunya-satunya yang dapat menolong memberi jalan keluar terbaik dari setiap urusan

• Tidak ada yang mustahil bagi-Nya, karena segala-galanya adalah milik-Nya, dan sepenuhnya dalam kekuasaan-Nya

• Tak ada yang dapat menghalangi jikalau Dia akan menolong hamba-hamba-Nya, Dialah yang mengatur segala sebab datangnya pertolongan-Nya

• Oleh karena itu aku harus benar-benar berjuang, berikhtiar untuk mendekati-Nya dengan mengamalkan apapun yang disukai-Nya dan melepaskan hati ini dari ketergantungan selain-Nya, karena selain Dia hanyalah sekedar makhluk yang tak berdaya tanpa kekuatan dari-Nya

• Ingatlah selalu janji-Nya :

“Barangsiapa yang bertaqwa kepada Allah, niscaya Ku beri jalan keluar dari setiap urusannya dan Kuberi rizki/ pertolongan dari tempat yang tak terduga, dan barangsiapa yang bertawakal kepada-Ku, Niscaya akan Kucukupi segala kebutuhannya”. ( At-Thalaq : 2-3 )

” Dan barangsiapa yang bertakwa kepada Allah niscaya Allah menjadikan baginya kemudahan dalam urusannya.” (At-Thalaq :4)

Semoga 5 kunci diatas dapat menenangkan hati yang sedang galau, cemas, was-was, khawatir yang berlebihan dan pengobat stress.
Ingat hanya dengan dzikrullah / mengingat Allah hati akan menjadi tenang.

Wassalam